Biscayne National Park offers guided boat tours on select Thursdays, Fridays, Saturdays, and Sundays that leave from the Dante Fascell Visitor Center. The boat tours, guided by the park, take place aboard the Pelican Skipper, a comfortable, 45-foot power catamaran. Visitors can delight in the spectacular scenery while learning about the ecosystems, wildlife, and history of Biscayne National Park. Each tour features a chance to explore the scenic and popular Boca Chita Key, as well as the park's historic and iconic lighthouse within the three hours. A boat tour ticket also grants visitors same-day admission to Homestead Bayfront Park.

Full-day sailing trips are offered from the Dante Fascell Visitor Center through Island Dreamer Sailing. During the six hour trip, passengers have the opportunity for island hiking, sailing, swimming, paddle boarding, kayaking, and snorkeling in Biscayne National Park. Passengers also have the chance to learn about the flora and fauna, dolphins, sea turtles, and manatees that live in the waters of Biscayne Bay.